Keywords: HeLa cell line expressing eGFP was generated by culture, analysis, and selection of clonal colonies on a PS-AA microraft array..jpg en HeLa cell line expressing eGFP was generated by culture analysis and selection of clonal colonies on a PS-AA microraft array A Brightfield and B fluorescence images of a region of an array microraft size 100 μm inter-raft spacing 20 μm height 15 μm containing clonal colonies of HeLa cells 7 days after gene transfection A single microraft with a colony composed entirely of eGFP-expressing cells is present C and D Images of the colony seen in A and B after release and collection E and F Images of the same colony 72 h after selection The fluorescent daughter cells are seen to be dividing and growing off the isolated microraft G and H Images of the same colony 6 days after selection A colony of ~500 cells in which all progeny are fluorescent has formed from the original cell 2013-03-27 15 57 23 http //www ncbi nlm nih gov/pmc/articles/PMC2994190/ Yuli Wang Colleen Phillips Wei Xu Jeng-Hao Pai Rahul Dhopeshwarkar Christopher E Sims and Nancy Allbrittona PD-USGov Uploaded with UploadWizard HeLa cells |